CJC-1295 without Drug Affinity Complex (Modified GRF 1-29)
Also Known AsMod GRF 1-29 · Modified GRF(1-29) · Tetrasubstituted GRF(1-29)
Modified analog of the first 29 amino acids of endogenous growth hormone-releasing hormone (GHRH). Four amino acid substitutions (positions 2, 8, 15, 27) were introduced to prevent enzymatic degradation by dipeptidyl peptidase-IV (DPP-IV) and improve metabolic stability. Developed to retain full GHRH receptor agonist activity with extended half-life relative to native GRF(1-29).
Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH2How CJC-1295 (No DAC) Works
GHRH Receptor Agonism
Binds the GHRH receptor (GHRHR) on anterior pituitary somatotroph cells, activating the Gs-adenylyl cyclase-cAMP-PKA signaling cascade. This stimulates transcription and release of growth hormone in a pulsatile manner, preserving the physiological GH secretory pattern rather than producing continuous elevation.
GH-IGF-1 Axis Stimulation
By amplifying endogenous GH pulses, CJC-1295 (No DAC) drives hepatic IGF-1 synthesis through the GH receptor–JAK2–STAT5b signaling pathway. The resulting elevation in circulating IGF-1 mediates many of the downstream anabolic and metabolic effects attributed to GH axis activation.
Somatostatin Sensitivity Preservation
Unlike exogenous GH administration, GHRH-based stimulation preserves the negative feedback loop mediated by somatostatin (SRIF). This maintains the normal pulsatile pattern of GH release and prevents the continuous GH elevation associated with exogenous GH administration.
Published Research
Growth Hormone Secretion Physiology
StrongWell-characterized pharmacology demonstrates dose-dependent amplification of GH pulse amplitude without disrupting pulse frequency or the somatostatin-mediated interpulse trough. GH response is synergistically enhanced when co-administered with GHRP-type secretagogues (e.g., ipamorelin) due to complementary receptor mechanisms.
Body Composition
ModerateStudies of GHRH analogs in GH-deficient and aging populations demonstrate improvements in lean body mass and reductions in visceral adiposity when GH axis output is restored to youthful levels. Effects are mediated through GH-stimulated lipolysis and IGF-1-driven protein synthesis.
Sleep Architecture & GH Secretion
ModerateEndogenous GH release is concentrated during slow-wave sleep (SWS). GHRH receptor activation has been shown to increase SWS duration in clinical studies of GHRH analogs, suggesting bidirectional interactions between the somatotropic axis and sleep regulatory circuits in the hypothalamus.
Age-Related GH Decline (Somatopause)
ModerateAge-related decline in GH secretion is primarily driven by increased somatostatin tone and reduced GHRH output rather than somatotroph failure. GHRH analogs can restore GH pulse amplitude in aged subjects, suggesting residual pituitary capacity. Research explores whether GH axis restoration confers metabolic and functional benefits.
Safety Profile
GHRH analogs have been studied extensively in clinical research with a well-characterized safety profile. Common adverse effects include transient injection site reactions, facial flushing, and headache. GH axis stimulation carries theoretical risks of GH/IGF-1 excess if dosing is not appropriately managed. Preserving pulsatile secretion (vs. continuous GH) reduces risks relative to exogenous GH administration.
Handling & Storage
Reconstitute with bacteriostatic water. Direct gentle stream along vial wall — do not shake. Store lyophilized powder at -20°C. Reconstituted solution at 2-8°C, use within 21 days. Sensitive to heat degradation; keep cold-chain intact during shipping.
Peer-Reviewed Literature
- 1
Pulsatile secretion of growth hormone (GH) persists during continuous stimulation by CJC-1295, a long-acting GH-releasing hormone analog
Ionescu M, Frohman LA.
- 2
Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, a long-acting analog of GH-releasing hormone, in healthy adults
Teichman SL, Neale A, Lawrence B, et al.
- 3
Once-daily administration of CJC-1295, a long-acting growth hormone-releasing hormone (GHRH) analog, normalizes growth in the GHRH knockout mouse
Alba M, Fintini D, Sagazio A, et al.
CJC-1295 (No DAC) FAQ
What is CJC-1295 (No DAC)?
CJC-1295 (No DAC) — also called Mod GRF(1-29) — is a modified 29-amino-acid analog of growth hormone-releasing hormone (GHRH). Four amino acid substitutions protect it from rapid enzymatic degradation while preserving full GHRH receptor agonist activity. 'No DAC' distinguishes it from the DAC-conjugated version that binds albumin for extended duration.
What is the difference between CJC-1295 with DAC and without DAC?
The DAC (Drug Affinity Complex) version includes a maleimidopropionic acid–lysine linker that covalently binds serum albumin in vivo, extending half-life to ~8 days and producing sustained GH elevation. The No DAC version (Mod GRF 1-29) has a shorter half-life (~30 minutes), producing acute GH pulse amplification that more closely mimics physiological secretion patterns.
Why is CJC-1295 (No DAC) often paired with Ipamorelin?
CJC-1295 (No DAC) activates the GHRH receptor while Ipamorelin activates the GHS-R1a (ghrelin) receptor. These are distinct, complementary pathways on pituitary somatotrophs. Co-stimulation produces a synergistic GH release that exceeds the sum of either agent alone, while both preserve physiological pulsatility.
How should CJC-1295 (No DAC) be stored?
Store lyophilized powder at -20°C for long-term stability. Reconstitute with bacteriostatic water and store at 2-8°C; use reconstituted solution within 21 days. This peptide is sensitive to heat — maintain cold-chain integrity.
CJC-1295 (No DAC) Products
All compounds 99%+ purity, verified by Janoshik Analytical. GMP-manufactured lyophilized powder.
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Disclaimer: This monograph is provided for educational and research purposes only. G26x Peptides products are sold exclusively as research chemicals. They are not intended for human consumption, therapeutic use, or as dietary supplements. All research should be conducted in compliance with applicable laws and institutional review board protocols. Information presented here is sourced from published peer-reviewed literature and does not constitute medical advice.